| NBID42 |
Functional Genomics , 6 ECTS credits.
/Functional Genomics/
For:
Bio
MOL
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Prel. scheduled
hours: 60
Rec. self-study hours: 100
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Area of Education: Science
Subject area: Biology
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Advancement level
(G1, G2, A): A
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Aim:
The course aims to give the student basic knowledge in the area of plant functional genomics, born in 2000 when the first complete genome of a flowering plant (Arabidopsis thaliana) has become available for large-scale functional analysis. The course will provide fundamental knowledge of the following technologies*: full length cDNA, transcriptomics, proteomics, metabolomics, T-DNA mutagenesis, yeast two-hybrid system as well as the basics of computer-based tools used for gene annotation in Arabidopsis thaliana.
After the course each student should be able to:
- explain the principles of each technology* used for functional analysis
- to carry out experimentally those included in the laboratory exercises
- critically discuss results obtained with various technologies* in selected research articles
- perform searches for literature and sequence-related information at various plant databases
- to answer a scientific question using the outcome of the above searches
- communicate orally and written his/her results
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Prerequisites: (valid for students admitted to programmes within which the course is offered)
Plant Molecular Genetics. Genomics and Bioinformatics or equivalent
Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding.
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Organisation:
The course will consist of lectures, discussion of scientific literature, and individual bioinformatics assignments. Exercises at commonly used servers and extensive searches in plant databases will also be trained. Attendance to the datalabs and seminars is compulsory.
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Course contents:
The course presents an overview of technologies* used for identifying and understanding plant gene function in the era of genomics. Applications of these technologies for several plants and plant-related species (Synechocystis, Chlamydomonas) as well as for particular protein families will also be discussed.
The course will also provide basic theoretical knowledge and hands on experience in using plant specific databases.
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Course literature:
Text book: Leister: Plant Functional Genomics (2005) Haworth Press
Research articles and reviews
Databases: TAIR: http://www.arabidopsis.org/, MIPS: http://mips.gsf.de/proj/plant/jsf/athal/, ARAMEMNON: http://aramemnon.botanik.uni-koeln.de/, PPDB (http://ppdb.tc.cornell.edu/, SIGnAL: http://signal.salk.edu/cgi-bin/tdnaexpress, RIKEN: http://www.brc.riken.jp/lab/epd/Eng/, Chlamydomonas Center: http://www.chlamy.org/, Cyanosite: http://www-cyanosite.bio.purdue.edu/index.html
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Examination: |
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Written examination Seminars and datalabs Individual assignment |
3 ECTS 2 ECTS 1 ECTS
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Course language is English.
Department offering the course: IFM.
Director of Studies: Agneta Johansson
Examiner: Cornelia Spetea-Wiklund
Link to the course homepage at the department
Course Syllabus in Swedish
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