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| TVCB01 | Methods in Structural Biology, 4 p (sw) /Strukturbiologiska metoder/ Advancement level: C | |
| Aim: The goal of the course is to give basic knowledge on the use of different methods to study the structure of biological molecules, cells and tissue.Prerequisites: Biochemistry (TFKE 25) and Cellbiology (TFBI 10).Course organization: Lectures, demonstrations and laboratory work.Course content: Preparation of tissue for microscopy: fixation, embedding, cutting, mounting of sections and staining. Construction of the electronmicroscope: different types of electronmicroscopes. Studying of biological tissue in the electronmicroscope. Freeze etching and freeze fracturing. Examination of biomolecules: NMR, x-ray crystallography and circular dicroism. Biological markers: Immunocytochemical methods. How to synthesize monoclonal and polyclonal antibodies. Autoradiography for light microscopy and electron microscopy. Quantitative analysis: Cytometry, including flow cytometry.Course literature: Lars-Inge Larsson: Imunocytochemistry-Theory and Practice, CRC Press. Bo Forslind: Elektronmikroskopi-Teori och Praktik, Liber förlag. M.G. Ormerod: Flow Cytometri-A Practical Approach, ISBN 0-19-963053-4. Articles and compendiums will cover the rest of the contents of the course. | ||
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