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| NKEC52 | Biochemistry 2, 10 p (sw) /BIOKEMI 2/ Advancement level: C | |
| Aim: The course provides comprehensive knowledge in the following areas in biochemistry: Structure and function of proteins, enzymology, bioenergetics, metabolism, structure and function of nucleic acids, molecular genetics. The laboratory course will give training in basic laboratory techniques and illustrate various theoretical aspects. Prerequisites: Approved General Chemistry 1-3, attended NKEB21 Inorganic Chemistry 2, NKEB22 Organic Chemistry 2 and attended courses in biology, 40p corresponding Biology 1 and 2. Supplementary courses: NKED74 Proteinchemistry, NKEC61 Gene TechnologyCourse organization: The theory is mainly presented on lectures. Solving of problems, discussions of theoretical and practical aspects of experiments in the laboratory course, studies of molecular models, computer simulations of protein purification are performed in smaller groups. Theoretical and practical aspects of biochemistry are illustrated in the laboratory course. The results of the experimental work should be presented and discussed in reports and at seminars. Course content: Theoretical part: Structure and function of biomolecules. The cell. Protein purification. Primary-, secondary-, tertiary- and quartenary structure. Protein folding, enzyme kinetics with different forms of inhibition, two-substrate kinetics. Enzyme catalysis, characterisation of enzyme mechanisms, examples of specific mechanisms. Bioenergetics, glycolysis, citric acid cycle, oxidative phosphorylation, photosynthesis, gluconeogenesis, lipid metabolism, amino acid metabolism, control mechanisms. Structure of nucleic acids, molecular genetics, replication, transcription, translation, gene control, eukaryotic and prokaryotic genes, gene technology, molecular immunology and molecular evolution. Experimental part: Protein purification: Centrifugation, osmolysis, precipitation, dialysis, ionic exchange chromatography. Purity and function analyses of enzymes: Isoelectric focusing, assay of enzyme activity, chromatogram, atomic absorption analysis, preparation of apoenzyme, role of cofactors, kinetic and spectrophotometric studies. Two-substrate kinetics. Microbiological work and gene technology experiments Course literature: Zubay, Parson, Vance: Principles of Biochemistry Laboratory manual from the department | ||
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