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| NKEB44 | Analytical Chemistry 2 , 10 p (sw) /ANALYTISK KEMI 2/ Advancement level: B | |
| Aim: The aim of the course is to give fundamental theoretical and practical knowledge in the field of modern analytical chemistry including both instrumental and non-instrumental techniques. Prerequisites: Approved NKEA14-15 General chemistry 1-2, NKEA16 Organic Chemistry 1, NKEA17 Biochemistry 1 and attended NKEB 21 Inorganic Chemistry 2 and NKEB 22 Organic Chemistry 2 (or equivalent). Supplementary courses: NKEC72 Organic Analytical Chemistry, NKEC65 Chemistry of Natural Waters with Trace Elemental AnalysisCourse organization: The course consists of lectures, laborations, short written tests and laboratory seminars. Course content: Quantitative analysis based on acid-base, complexometric and redox titrations. Fundamentals of electrochemistry. Electrochemical methods including electrodes and potentiometry, coulometric analysis and voltammetry. Theory of chromatographic separation. Gas chromatography (GC) including sample injection, separation and detection. High performance liquid chromatographic (HPLC) methods such as normal phase and reverse phase HPLC, size-exclusion chromatography and ion chromatography. Introduction to mass spectrometry (MS) and GC-MS. Molecular ultraviolet/visible absorption spectroscopy and molecular fluorescence. Atomic spectroscopy based upon flame and electrothermal atomization, emission spectroscopy based on plasma sources (ICP). Experimental error and elementary statistics. Course literature: D.C. Harris: Quantitative Chemical Analysis. W.H. Freeman and Company. R.D. Beaty and J.D. Kerber: Concepts, Instrumentation and Techniques in Atomic Absorption Spectrophotometry. The Perkin-Elmer Corporation. Complementary material. | ||
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