| TFKE36 |
Biochemistry, 6 ECTS credits.
/Biokemi 2/
For:
Bio
KA
KeBi
Kem
TB
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Prel. scheduled
hours: 46
Rec. self-study hours: 114
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Area of Education: Science
Main field of studies: Chemistry
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Advancement level
(G1, G2, A): G2
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Aim:
The objective of the couse is to give comprehensive knowledge within the following areas: Enzyme kinetics, enzyme mechanisms and methodology for characterization of enzymes.After studies well learned the student will have proficiency to:
- Calculate kinetic parameters like kcat, KM, Vmax and KI.
- Interprete the meaning of kinetic parameters in terms of enzyme function.
- Recapitulate the reaction mechanism for some enzymes.
- Explore fundamental laboratory techniques and interprete the obtained data in a written report.
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Prerequisites: (valid for students admitted to programmes within which the course is offered)
General chemistry, Organic chemsitry 1 and Biochemistry 1
Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding.
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Supplementary courses:
Protein Chemistry
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Organisation:
The theory is mainly presented in lectures. Solving of problems, discussions of theoretical and practical aspects of experiments in the laboratory are performed in lessons. Theoretical and practical aspects of biochemistry are illustrated in the laboratory course. The results of the experimental work should be presented and discussed in written reports.
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Course contents:
Theory: Structure and function of enzymes. Protein folding, enzyme kinetics with different types of inhibitions. Factors causing enzyme catalysis, characterization of the active site, examples of the reation mechansims of certain enzymes. Laborations: Purification of a protein with ionic change chromatography. Functional analysis of enzymes: Enzyme activity measurement, pH-dependance, chromatogram, kinetic- and spectrophotometric studies.
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Course literature:
Berg, Tymoczko & Stryer: Biochemistry 7th or 8th edition. Laboratory manual from the department.
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Examination: |
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A written examination
Laboratory course
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4 ECTS
2 ECTS
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The exercises of the written examination and the test elucidate how well the student perform the the demands of the course |
Course language is Swedish.
Department offering the course: IFM.
Director of Studies: Magdalena Svensson
Examiner: Magdalena Svensson
Course Syllabus in Swedish
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