Study Guide@lith
 

Linköping Institute of Technology

 
 
Valid for year : 2016
 
TFKE54 Preparation of Proteins, 3 ECTS credits.
/Proteinpreparation/

For:   Bio   KeBi   Kem   PRO  

 

Prel. scheduled hours: 34
Rec. self-study hours: 46

  Area of Education: Technology

Main field of studies: Biology, Chemical Biology

  Advancement level (G1, G2, A): A

Aim:
The objective of the course is to provide comprehensive knowledge in the following areas: Protein chemistry, expression of cloned proteins and methodologies for protein purification as well as experimental planning. After studies well learned the student will have proficiency to:
  • Express and purify cloned proteins
  • Adopt a reflecting, scientific attitude to theories and obtained experimental results.


Prerequisites: (valid for students admitted to programmes within which the course is offered)
Protein Chemistry

Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding.

Organisation:
The course has a pronounced experimental focus and character. Work is done on genetically mutated human proteins cloned in bacteria. The origin of the mutated proteins can be proteins that have been mutated by the students in an earlier course in gene technology or can be provided by the research group. The protein variants are expressed in E.coli and purified by affinity chromatography. Purity and yield are determined. The students should then independently evaluate their experimental data. The project is reported as a report.

Course contents:
During the course methods such as expression of cloned proteins, affinity chromatography and SDS-PAGE are used. Various spectroscopic techniques such as absorbance, fluorescence and circular dichroism are used.

Course literature:
Scientific articles. Compendium.

Examination:
Project
3 ECTS
 
Grades are given as �?TFail�?T or �?TPass�?T.



Course language is Swedish.
Department offering the course: IFM.
Director of Studies: Magdalena Svensson
Examiner: Lars-Göran Mårtensson

Course Syllabus in Swedish

Linköping Institute of Technology

 


Contact: TFK , val@tfk.liu.se
Last updated: 10/23/2015