| TFKE38 |
Gene Technology, 3 ECTS credits.
/Genteknik/
For:
KeBi
PRO
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OBS! |
Canā?Tt be combined with other courses Ht1 due to special scheduling
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Prel. scheduled
hours: 42
Rec. self-study hours: 38
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Area of Education: Technology
Main field of studies: Chemical biology
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Advancement level
(G1, G2, A): G2
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Aim:
The course will give fundamental knowledge and experimental skills in the following areas of Molecular Biology: Site-specific mutagenesis, gene cloning and PCR After studies well learned the student will have proficiency to:
- Understand basic laboratory techniques in molecular biology techniques to perform site-directed mutagenesis, amplification of DNA fragments using PCR and cloning of DNA fragments
- Describe different types of vectors for cloning and expression of eukaryotic genes in a prokaryotic system
- Theoretical knowledge of DNA sequencing
- Theoretical and practical knowledge of techniques for purification of plasmid DNA
- Describe the function and applications of different enzymes involved in gene cloning experiments
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Prerequisites: (valid for students admitted to programmes within which the course is offered)
Biochemistry, first and second course
Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding.
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Supplementary courses:
Protein chemistry
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Organisation:
The theory is mainly presented in lectures. Solving of problems, discussions of theoretical and practical aspects of experiments in the laboratory are performed in lessons. Theoretical and practical aspects are illustrated in the laboratory course. The results of the experimental work should be presented and discussed in written reports. Laboration course in site-directed mutagenesis.
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Course contents:
Purifications of DNA, manipulation of DNA, cloning of genes into E.coli vectors, control of gene expression, differences in prokaryotic and eukaryotic genes, mutation analysis, DNA-sequencing.
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Course literature:
T.A. Brown Gene Cloning and DNA analysis, 6th edition.
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Examination: |
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Written examination
Laboratory work
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2 ECTS
1 ECTS
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Course language is English.
Department offering the course: IFM.
Director of Studies: Magdalena Svensson
Examiner: Lars-Göran Mårtensson
Course Syllabus in Swedish
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