| NKED80 | Combinational Protein Engineering, 6 ECTS credits. /Kombinatorisk proteinteknik/ |
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Prel. scheduled
hours: 60 |
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Area of Education: Science Subject area: Chemical Biology/Chemistry |
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| Advancement level
(G1, G2, A): A
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| Aim: The course goal is to give the students a deep knowledge about methods that are used in modern protein technology. Special emphasis is put on methods for generation of novel protein functions using in vitro evolution. After the course the student is expected to: �Y Master important concepts, methods and theories that are used for design and redesign of proteins �Y Have good skills to communicate research problems, methods and results within the field of combinatorial protein technology �Y Have the ability to critically read advanced literature within the field. |
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| Prerequisites: (valid for students admitted to programmes within which the course is offered) Gene Technology and Protein Chemistry Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding. |
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| Organisation: The course will contain a mix of lectures, seminars and laboratory work. The laboratory work is performed individually or in a group. All educational subsections are compulsory. |
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| Course contents: The course includes methods for design and redesign of proteins with new properties and functions by use of in vitro evolution. Phage display, ribosome display, DNA-shuffling and error prone PCR and other combinatorial methods are penetrated. The course also includes methods for redesign of proteins using directed mutagenesis and modular approaches for construction of fusion proteins. A large section treats methods for isolation of proteins with defined properties and functions by screening or by selection. |
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| Course literature: Original scientific articles and reviews and a textbook that is not yet determined. |
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| Examination: | ||||
| Written examination Laboratory work |
2 p 2 p |
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3 ECTS 3 ECTS |
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Course language is Swedish. Department offering the course: IFM. Director of Studies: Stefan Svensson Examiner: Course Syllabus in Swedish |
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