| TFKE54 |
Preparation of Proteins, 3 ECTS credits.
/Proteinpreparation/
For:
Bio
KeBi
Kem
KOS
PRO
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Prel. scheduled
hours: 34
Rec. self-study hours: 46
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Area of Education: Technology
Main field of studies: Biology, Chemical Biology
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Advancement level
(G1, G2, A): A
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Aim:
The objective of the course is to provide comprehensive knowledge in the following areas: Protein chemistry, expression of cloned proteins and methodologies for protein purification as well as experimental planning. After studies well learned the student will have proficiency to:
- Express and purify cloned proteins
- Adopt a reflecting, scientific attitude to theories and obtained experimental results.
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Prerequisites: (valid for students admitted to programmes within which the course is offered)
Protein Chemistry
Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding.
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Organisation:
The course has a pronounced experimental focus and character. Work is done on genetically mutated human proteins cloned in bacteria. The origin of the mutated proteins can be proteins that have been mutated by the students in an earlier course in gene technology or can be provided by the research group. The protein variants are expressed in E.coli and purified by affinity chromatography. Purity and yield are determined. The students should then independently evaluate their experimental data. The project is reported as a report.
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Course contents:
During the course methods such as expression of cloned proteins, affinity chromatography and SDS-PAGE are used. Various spectroscopic techniques such as absorbance, fluorescence and circular dichroism are used.
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Course literature:
Scientific articles. Compendium.
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Examination: |
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Project
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3 ECTS
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Grades are given as â?TFailâ?T or â?TPassâ?T. |
Course language is Swedish/English.
Department offering the course: IFM.
Director of Studies: Magdalena Svensson
Examiner: Lars-Göran Mårtensson
Course Syllabus in Swedish
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