| NBID37 | Functional Genomics - Applications in Plants, 6 ECTS credits. /Functional Genomics - Applications in Plants/ |
||
Prel. scheduled
hours: 60 |
|||
|
Area of Education: Science Subject area: Biology |
|||
| Advancement level
(G1, G2, A): A
|
|||
| Aim: The course aims to give the student basic knowledge in the area of plant functional genomics, born in 2000 when the first complete genome of a flowering plant (Arabidopsis thaliana) has become available for large-scale functional analysis. The course will provide fundamental knowledge of the following technologies*: full length cDNA, transcriptomics, proteomics, metabolomics, T-DNA mutagenesis, yeast two-hybrid system as well as the basics of computer-based tools used for gene annotation in Arabidopsis thaliana. Learning outcomes: After the course each student should be able to:
|
|||
| Prerequisites: (valid for students admitted to programmes within which the course is offered) Plant Molecular Genetics Genomics and Bioinformatics or equivalent Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding. |
|||
| Organisation: The course will consist of lectures, discussion of scientific literature, a practical demonstration in mass spectrometry-based proteomics and one laboratory. Exercises at commonly used servers and extensive searches in plant databases will also be trained. Attendance to the data and wet labs, practical demonstration and seminars is compulsory. |
|||
| Course contents: The course presents an overview of technologies* used for identifying and understanding plant gene function in the era of genomics. Applications of these technologies for several plants and plant-related species (Synechocystis, Chlamydomonas) as well as for particular protein families will also be discussed. The course will also provide basic theoretical knowledge and hands on experience in using plant specific databases. During the laboratory work, the students will obtain experience in screening for homozygotes T-DNA insertion Arabidopsis mutants. A practical demonstration in mass spectrometry-based plant proteomics at LiU/HU is integrated in the course. |
|||
| Course literature: Text book: Leister: Plant Functional Genomics (2005) Haworth Press Research articles and reviews Databases: TAIR: http://www.arabidopsis.org/ MIPS: http://mips.gsf.de/proj/plant/jsf/athal/ ARAMEMNON: http://aramemnon.botanik.uni-koeln.de/ PPDB (http://ppdb.tc.cornell.edu/ SIGnAL: http://signal.salk.edu/cgi-bin/tdnaexpress RIKEN: http://www.brc.riken.jp/lab/epd/Eng/ Chlamydomonas Center: http://www.chlamy.org/ Cyanosite: http://www-cyanosite.bio.purdue.edu/index.html |
|||
| Examination: | |||
| Written examination Seminars and datalabs Individual assignment |
3 ECTS 2 ECTS 1 ECTS |
||
Course language is English. Department offering the course: IFM. Director of Studies: Agneta Johansson Examiner: Cornelia Spetea-Wiklund Link to the course homepage at the department Course Syllabus in Swedish |
|||
|
|
 |
|||||
| ||||||