| TVCB02 | Gene Technology and Molecular Genetics, 6 ECTS credits. /Genteknik och molekylärgenetik/ |
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Prel. scheduled
hours: 63 |
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Area of Education: Science Subject area: Biology/Chemistry |
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| Advancement level
(A-D): C
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| Aim: The course should provide theoretical and practical knowledge in molecular genetic technologies and their use in the biomedical field. The course should also illuminate the use of databases covering genetic information. |
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| Prerequisites: (valid for students admitted to programmes within which the course is offered) Biochemistry (TFKE 25) and Cell Biology (TFBI 10). Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding. |
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| Organisation: The course consists of lectures, seminars and laboratory work. |
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| Course contents: DNA: Control of gene expression in eukaryotic cells. DNA damage and repair. Medical genetics and the HUGO project. Genetic variation, polymorphism and mutation. Cytogenetics. Basic gene technologies: Restriction enzymes and RFLP. Separation of nucleic acids. Immobilization on membranes (Northern and Southern blotting). Labeling and hybridization. Nucleotide sequencing. Isolation of genes (cloning) and use of vectors. PCR. Use of computers in molecular genetic work (sequence databases). Applications of gene technologies in biomedicine: Clinical diagnostics, linkage analysis, prenatal diagnostics and genetic counseling. Applications in forensics. |
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| Course literature: Alberts B. et al: Molecular Biology of the Cell, Garland Publ. Co., New York and London, 4th ed, 2002. Strachan, Tom & Read, Andrew P.: Human Molecular Genetics, BIOS Scientific Publishers Ltd, Oxford, UK, 3rd ed, 2002. |
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| Examination: | |||
| Written examination Labratory work Written examination |
2 p 1 p 1 p |
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Course language is Swedish. Department offering the course: IBK. Director of Studies: Ingemar Rundquist Examiner: Peter Söderkvist Course Syllabus in Swedish |
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