| NKEC12 | Gene Technology, 7,5 ECTS credits. /Genteknik/ |
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Prel. scheduled
hours: 60 |
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Area of Education: Science Subject area: Chemistry |
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| Advancement level
(A-D): C
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| Aim: The course will give fundamental knowledge and experimental skills in the area of Recombinant-DNA techniques and microbiology. |
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| Prerequisites: (valid for students admitted to programmes within which the course is offered) Attended NKEC52 Biochemistry 2 Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding. |
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| Supplementary courses: NKED74 Protein Chemistry |
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| Organisation: The course is laboratory intense and will give experimental training in techniques used in microbiology and gene cloning experiments. Seminars and lecures are intended to give the theoretical background using Recombinant-DNA techniques. |
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| Course contents: Theory The following areas are treated during the lectures: Preparation methods for nucleic acids, purifying DNA-fragment, labeling of nucleic acid probes, enzymes involved in gene cloning, plasmidbiology, various vector system for cloning and expression of proteins, protein engineering including site-directed mutagenesis and random mutagenesis, Mutation analysis, DNA-sequencing. Experimental Transformation of plasmids using competent cells, isolation and purification of plasmids, analysis of plasmid-DNA using agarose gel electrophoresis, isolation of SS-DNA for mutagenesis, site-directed mutagenesis, DNA-sequencing, expression and purification of cloned mutated proteins in bacterial cells. |
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| Course literature: T.A Brown Gene Cloning and DNA analysis, 4th edition Laboratory manuals Scientific articles |
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| Examination: | |||
| Hand-in assignment Laboratory course with final seminar |
2 p 3 p |
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Course language is . Department offering the course: IFM. Director of Studies: Stefan Svensson Examiner: Lars-Göran Mårtensson Course Syllabus in Swedish |
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