TFBI13 Molecular Genetics 2007

Study Guide@lith

Valid for year : 2007

TFBI13	Molecular Genetics, 6 ECTS credits. /Molekylärgenetik/ For: KeBi
	Prel. scheduled hours: Rec. self-study hours: 160
	Area of Education: Science Subject area: Chemical biology/Biology
	Advancement level (G1, G2, A): G2
	Aim: Procaryotic molecular and cell biology have been at the forefront of fundamental discoveries in molecular biology for last 40 years. Bacteria are perfect model organisms because of small genome and rapid growth rate. Studies with bacteria resulted in recent progress in the understanding of the regulation of complex biological processes. Bacteria live in changing environment and must respond to this. They employ unique strategies to survive during various conditions. After finished course the students can: present advances in the knowledge about the molecular mechanisms of the procaryotic cell regulation and answer the question: How bacteria respond to changing environments? present novel regulatory systems discovered in recent years. discuss the common procaryotic signal transduction mechanisms and the metabolic controls that rely on the ability of cells to accurately respond to the levels of nutrients and product in the cell and in the environment. understand novel regulatory system using cyclic nucleotides (c-di GMP). discuss recent results concerning controls at the level of translation and degradation by small RNAs. Small RNAs represent the key regulators of environmental adaptation and virulence-gene expression.
	Prerequisites: (valid for students admitted to programmes within which the course is offered) Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding.
	Organisation: Lectures, seminars and discussion of recent literature.
	Course contents: Overview of regulatory mechanisams: Operons under promoter control, operons under attenuator control, operon regulation by translational control, catabolite repression, the stringet response. Regulation of gene expression by sigma factors. Regulation of gene expression via signal transduction pathways. Epigenetic gene regulation in the bacteria. Role of DNA Adenine methylation in regulating bacterial gene expression and virulence. RNA molecules in gene regulation: Riboswitches and the role of noncoding RNAs in gene control. Small RNAs, coordinators of adaptation processes in bacteria. The role of RNAs in the regulation of virulence-gene expression.
	Course literature: Singleton Paul: Bacteria in Biology, Biotechnology and medicine 5th edition, John Wiley and Sons, LTD Madigan M.T., Martinko J.M.:Brock Biology of Microorganisms 11ed Pearson, Prentice Hall Actual articles: Wion, D., Casadesus, J 2006 N6-methyl-adenine: an epigenetic signal för DNA-protein intereaktions Nature Revievs Microbiology Vol 4:183-192. Low, D.A., Weyand, N. J., and Mahan, M.J.2001. Roles of DNA Adenine methylation in regulating bacterial gene expression and virulence . Infection and Immunity, Vol 69 : 7197-7204. Casadeus, J. Low, D. 2006 Epigenetic Gene Regulation in the Bacterial World. Microbiolofgy and Molecular Biology Reviews, Vol 70: 830-856. Tucker, B.J., Breaker, R.R.2005. Riboswitches as versatile gene control elements. Current Opinion in Structural Bilology, Vol.15:342-348. Winkler, W.C.2005.Riboswitches and the role of noncoding RNAs in bacterial metabolic control. Current Opinion in Chemical Biology. Vol 9:594-602. Storz G.,Haas, D. 2007. A guide to small RNAs in microorganisms. Current Opinion in Microbiology. Vol 10:93-95. Gottesman, S. 2005. Micros for microbes:non-coding regulatory RNAs in bacteria. Trends in Genomics.Vol.2:399-404. Storz,G., Opdyke, J.,A.,Zhang, A. Controlling mRNA stability and translation with small. noncoding RNAs. Current Opinion in Microbiology, Vol7:140-144. Repoila, F.Majdalani, N., Gottesman, S. 2003. Small non-coding RNAs, co-ordinators of adaptation processes in Escherichia coli: the RpoS paradigm. Molecular Microbiology, Vol.48: 855-861. Wasserman, K.,M. 2002. Small RNAs in bacteria: Diverse regulators of gene expression in response to environmental changes. Cell, Vol 109:141-144. Toledo-Arana,A., Repoila, F., Cossart, P. 2007: Small noncoding RNAs controlling pathogenesis.Current Opinion in Microbiology, Vol10:182-188. Romby, P., Vandenesch, F., Wagner, G.,H. The role of RNA in the regulation of virulence-gene expression. Current Opinion in Microbiology, Vol.9:2219-236. Complement literature Articles of students individual choice
	Examination:
	Written examination	4 p	/	6 ECTS


Course language is . Department offering the course: IFM. Director of Studies: Agneta Johansson Examiner: Course Syllabus in Swedish

Linköping Institute of Technology
Contact: TFK , val@tfk.liu.se Last updated: 12/11/2007