| NBID35 | Immunological Techniques, 6 ECTS credits. /Immunological Techniques/ |
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Prel. scheduled
hours: 60 |
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Area of Education: Science Subject area: Biology |
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| Advancement level
(G1, G2, A): A
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| Aim: The course intends to provide a basic understanding of the use of antibodies in life science research and specific knowledge and practical skills in three different immunological techniques*; immunohistochemistry, ELISA and Western blotting. After the course each student should be able to:
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| Prerequisites: (valid for students admitted to programmes within which the course is offered) A bachelor's degree with a major in biology including advanced courses in molecular biology or related subjects, at least 24 ECTS credits. Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding. |
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| Organisation: The course will include lectures, seminars and laboratory exercises. The results from the practical work will be presented orally and in a written report. Attendance to the labs and seminars is compulsory. |
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| Course contents: The course focuses on three different immunological techniques; immunohistochemistry, ELISA and Western blotting. The students are expected to demonstrate both theoretical and practical knowledge about these three techniques. The course will provide theoretical background about histological "know how", light microscopy, protein separation and blotting and detection following polyacrylamide gel-electrophoresis. During the practical laboratory work, the students will obtain "hands on" experience with each of the three techniques*. Emphasis of the course will be to give understanding about why and when the different methods are suitable to use, and how to analyze and communicate the obtained results. |
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| Course literature: Scientific articles. Laboratory manuals produced at the department. |
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| Examination: | |||
| Approved laboratory course Written report Oral presentation |
2 ECTS 3 ECTS 1 ECTS |
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| Grades are given as �?TFail�?T or �?TPass�?T. | |||
Course language is English. Department offering the course: IFM. Director of Studies: Agneta Johansson Examiner: Thomas Östholm Link to the course homepage at the department Course Syllabus in Swedish |
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