NKEC12 Gene Technology 2007

Study Guide@lith

Valid for year : 2007

NKEC12	Gene Technology, 7,5 ECTS credits. /Genteknik/ For: BKM KeBi Kem
	Prel. scheduled hours: 60 Rec. self-study hours: 140
	Area of Education: Science Subject area: Chemistry
	Advancement level (G1, G2, A): G2
	Aim: The course will give fundamental knowledge and experimental skills in the following areas of Molecular Biology: Site-specific mutagenesis, gene cloning and PCR After studies well learned the student will have proficiency to: Experimentally use molecular biology techniques to perform site-directed mutagenesis, amplification of DNA fragments using PCR and cloning of DNA fragments Describe different types of vectors for cloning and expression of eukaryotic genes in a prokaryotic system Theoretical knowledge of DNA sequencing Theoretical and practical knowledge of techniques for purification of plasmid DNA Describe the function and applications of different enzymes involved in gene cloning experiments
	Prerequisites: (valid for students admitted to programmes within which the course is offered) Biochemistry 2 Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshhold requirements for progression within the programme, or corresponding.
	Supplementary courses: Protein Chemistry
	Organisation: The course is laboratory intense and will give experimental training in techniques used in microbiology and gene cloning experiments. Seminars and lectures are intended to give the theoretical background using Recombinant-DNA techniques.
	Course contents: Theory The following areas are treated during the lectures: Preparation methods for nucleic acids, purifying of DNA-fragments , enzymes involved in gene cloning, plasmidbiology, various vector system for cloning and expression of proteins, protein engineering including site-directed mutagenesis and random mutagenesis, Mutation analysis, DNA-sequencing. Experimental Transformation of plasmids using competent cells, isolation and purification of plasmids, analysis of plasmid-DNA using agarose gel electrophoresis, site-directed mutagenesis.
	Course literature: T.A Brown Gene Cloning and DNA analysis, 5th edition Laboratory manuals and Scientific articles
	Examination:
	Hand-in assignment Laboratory course with final seminar	2 p 3 p	/ /	3 ECTS 4,5 ECTS

	Grades are given as �?~Fail�?T or �?~Pass�?T.
Course language is Swedish. Department offering the course: IFM. Director of Studies: Stefan Svensson Examiner: Lars-Göran Mårtensson Course Syllabus in Swedish

Linköping Institute of Technology
Contact: TFK , val@tfk.liu.se Last updated: 12/12/2007